Over the previous decade, we’ve got witnessed an growing quantity of large-scale research which have supplied multi-omics information by high-throughput sequencing approaches. This has notably helped with figuring out key (epi)genetic alterations in cancers. Importantly, aberrations that result in the activation of signaling networks by way of the disruption of regular mobile homeostasis is seen each in most cancers cells and additionally within the neighboring tumor microenvironment.
Cancer methods biology approaches have enabled the environment friendly integration of experimental information with computational algorithms and the implementation of actionable focused therapies, because the exceptions, for the remedy of most cancers. Comprehensive multi-omics information obtained by way of the sequencing of tumor samples and experimental mannequin methods will probably be necessary in implementing novel most cancers methods biology approaches and growing their efficacy for tailoring novel personalised remedy modalities in most cancers.
In this overview, we talk about rising most cancers methods biology approaches based mostly on multi-omics information derived from bulk and single-cell genomics research along with current experimental mannequin methods that play a essential position in understanding (epi)genetic heterogeneity and remedy resistance in most cancers.
The finest predictions in experimental biology are essential and persuasive
A robust option to consider scientific explanations (hypotheses) is to check the predictions that they make. In this fashion, predictions function an necessary bridge between summary hypotheses and concrete experiments. Experimental biologists, nonetheless, typically obtain little steerage on easy methods to generate high quality predictions. Here, we establish two necessary elements of good predictions – criticality and persuasiveness – which relate to the power of a prediction (and the experiment it implies) to disprove a speculation or to persuade a skeptic that the speculation has benefit.
Using an in depth instance, we reveal how striving for predictions which are each essential and persuasive can pace scientific progress by main us to extra highly effective experiments. Finally, we offer a top quality management guidelines to help college students and researchers as they navigate the hypothetico-deductive methodology from puzzling observations to experimental checks.
Literature-based studying and experimental design mannequin in molecular biology educating for medical college students at Tongji University
In 2018, to assist undergraduate medical college students strengthen their self-learning and scientific analysis expertise, we launched an tutorial mannequin that mixed literature-based studying with experimental design. We examined this mannequin on the molecular biology class at Tongji University. In step one of the mannequin, a subject is chosen, and college students discover, learn, and consider scientific papers in teams and ship shows.
In the second step, they design scientific experiments in teams and talk about their proposed experiments at school, which is to be adopted by additional experimental verification within the lab course. This total exercise was given 20% weightage within the ultimate rating. The mannequin led to raised student-centered educating and self-directed studying in line with quantitative and qualitative evaluation. Students confirmed nice curiosity in literature and analysis.
They loved group work and gained expertise in group and presentation. Apart from a major improve in ultimate rating, evaluation information from college students indicated that they have been glad with this educating mannequin and thought-about it a optimistic expertise. Looking on the optimistic affect of the literature-based studying and experiment design mannequin, we help its continued use for educating molecular biology to undergraduate medical college students.
Increased Scaffolding and Inquiry in an Introductory Biology Lab Enhance Experimental Design Skills and Sense of Scientific Ability
We current a mannequin for the method of redesigning the laboratory curriculum in Introductory Organismal Biology to extend alternatives for significant inquiry and improve pupil recognition of their scientific ability growth. We created scaffolded modules and assignments to permit college students to construct and follow key expertise in experimental design, information evaluation, and scientific writing.
Using the Tool for Interrelated Experimental Design, we confirmed considerably larger features in experimental design scores within the redesigned course and a extra constant sample of features throughout a variety of preliminary pupil scores in contrast with the unique format. Students who accomplished the redesigned course rated themselves considerably larger in experimental design, information assortment, and information evaluation expertise in contrast with college students within the authentic format.
Scores on the Laboratory Course Activity Survey have been excessive for each codecs and didn’t considerably differ. However, on written course evaluations, college students within the redesigned course have been extra prone to report that they engaged in “actual science” and their “personal experiments.” They additionally had elevated recognition of their particular analytical and writing ability growth. Our outcomes reveal that intentional, scaffolded instruction utilizing inquiry modules can improve experimental design expertise and sense of scientific potential in an introductory biology course.
The ITGB6 gene: its position in experimental and medical biology
Integrin αvβ6 is a membrane-spanning heterodimeric glycoprotein concerned in wound therapeutic and the pathogenesis of ailments together with fibrosis and most cancers. Therefore, it’s of nice medical curiosity for us to grasp the molecular mechanisms of its biology.
 Human Mucin 2, MUC2 ELISA Kit |
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MBS705019-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 600 |
Human Mucin 2, MUC2 ELISA Kit |
|
MBS705019-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3105 |
Human Mucin 2, MUC2 ELISA Kit |
|
MBS705019-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 855 |
 ELISA Kit) Human Mucin 2(MUC2) ELISA Kit |
|
CSB-E13122h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
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|
|
Description: Quantitativesandwich ELISA kit for measuring Human Mucin 2 (MUC2) in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Mucin 2(MUC2) ELISA Kit |
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1-CSB-E13122h |
Cusabio |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 5 plates of 96 wells each
- 1 plate of 96 wells
- 10 plates of 96 wells each
|
|
|
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Description: Quantitativesandwich ELISA kit for measuring Human Mucin 2(MUC2) in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
 ELISA Kit) Human Mucin 2 (MUC2) ELISA Kit |
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AE32275HU-48T |
Abebio |
48T |
EUR 360 |
|
|
|
Description: Human (Homo sapiens) |
Human Mucin 2 (MUC2) ELISA Kit |
|
AE32275HU-48Tests |
Abebio |
48 Tests |
EUR 360 |
|
|
|
Description: Human (Homo sapiens) |
Human Mucin 2 (MUC2) ELISA Kit |
|
AE32275HU-96T |
Abebio |
96T |
EUR 680 |
|
|
|
Description: Human (Homo sapiens) |
Human Mucin 2 (MUC2) ELISA Kit |
|
AE32275HU-96Tests |
Abebio |
96 Tests |
EUR 680 |
|
|
|
Description: Human (Homo sapiens) |
Human Mucin 2 (MUC2) ELISA Kit |
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DLR-MUC2-Hu |
DL Develop |
96T |
EUR 422 |
|
|
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Description: tissue homogenates, cell lysates or other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
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DLR-MUC2-Hu-48T |
DL Develop |
48T |
EUR 574.8 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Mucin 2 (MUC2) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
|
DLR-MUC2-Hu-96T |
DL Develop |
96T |
EUR 745.2 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Mucin 2 (MUC2) in samples from tissue homogenates, cell lysates or other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
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DL-MUC2-Hu |
DL Develop |
96T |
EUR 402 |
|
|
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Description: tissue homogenates, cell lysates or other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
|
EK9084 |
SAB |
96Т |
EUR 799 |
|
|
Human Mucin 2 (MUC2) ELISA Kit |
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KTE61458-48T |
Abbkine |
48T |
EUR 359 |
|
|
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Description: This Human Mucin 2 (MUC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC2. |
Human Mucin 2 (MUC2) ELISA Kit |
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KTE61458-96T |
Abbkine |
96T |
EUR 619 |
|
|
|
Description: This Human Mucin 2 (MUC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC2. |
Human Mucin 2 (MUC2) ELISA Kit |
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KTE61458-96T5 |
Abbkine |
96 T×5 |
EUR 2809 |
|
Description: This Human Mucin 2 (MUC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC2. |
Human Mucin 2 (MUC2) ELISA Kit |
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KTE61458-96T50 |
Abbkine |
96 T×50 |
EUR 26209 |
|
Description: This Human Mucin 2 (MUC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC2. |
Human Mucin 2 (MUC2) ELISA Kit |
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MBS2702320-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 2830 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2702320-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 240 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2702320-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 315 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2702320-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 1480 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2702320-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 385 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS285830-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 6840 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS285830-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 480 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS285830-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3610 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS285830-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 805 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2021984-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 4910 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2021984-24StripWells |
MyBiosource |
24-Strip-Wells |
EUR 340 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2021984-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 500 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2021984-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2675 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS2021984-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 665 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS3801708-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 6725 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS3801708-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 550 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS3801708-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3420 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS3801708-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 765 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS4501206-10x96Tests |
MyBiosource |
10x96Tests |
EUR 4890 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS4501206-48Tests |
MyBiosource |
48Tests |
EUR 425 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS4501206-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2495 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS4501206-96Test |
MyBiosource |
96Test |
EUR 560 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS454927-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 4700 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS454927-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 410 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS454927-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 2410 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS454927-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 545 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS9715122-48Tests |
MyBiosource |
48Tests |
EUR 350 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS9715122-5x96Tests |
MyBiosource |
5x96Tests |
EUR 1940 |
Human Mucin 2 (MUC2) ELISA Kit |
|
MBS9715122-96Tests |
MyBiosource |
96Tests |
EUR 520 |
Human Mucin 2 (MUC2) ELISA Kit |
|
SEA705Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5128.02 |
|
|
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mucin 2 (MUC2) in tissue homogenates, cell lysates and other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
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SEA705Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 527.48 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mucin 2 (MUC2) in tissue homogenates, cell lysates and other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
|
SEA705Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 702.12 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mucin 2 (MUC2) in tissue homogenates, cell lysates and other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
|
SEA705Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2799.54 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mucin 2 (MUC2) in tissue homogenates, cell lysates and other biological fluids. |
Human Mucin 2 (MUC2) ELISA Kit |
|
4-SEA705Hu |
Cloud-Clone |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 1 plate of 96 wells
- 5 plates of 96 wells
- 10 plates of 96 wells
|
|
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Mucin 2 (MUC2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Mucin 2 (MUC2) ELISA Kit |
|
RDR-MUC2-Hu-48T |
Reddot Biotech |
48T |
EUR 454 |
|
|
|
Description: sandwich |
Human Mucin 2 (MUC2) ELISA Kit |
|
RDR-MUC2-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 600 |
Human Mucin 2 (MUC2) ELISA Kit |
|
RDR-MUC2-Hu-96T |
Reddot Biotech |
96T |
EUR 648 |
|
|
|
Description: sandwich |
Human Mucin 2 (MUC2) ELISA Kit |
|
RDR-MUC2-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 830.4 |
Human Mucin 2 (MUC2) ELISA Kit |
|
RD-MUC2-Hu-48T |
Reddot Biotech |
48T |
EUR 432 |
|
|
|
Description: sandwich |
Human Mucin 2 (MUC2) ELISA Kit |
|
RD-MUC2-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 573.6 |
Human Mucin 2 (MUC2) ELISA Kit |
|
RD-MUC2-Hu-96T |
Reddot Biotech |
96T |
EUR 617 |
|
|
|
Description: sandwich |
Human Mucin 2 (MUC2) ELISA Kit |
|
RD-MUC2-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 794.4 |
Human Mucin 2 (MUC2) ELISA Kit |
|
RK09085 |
Abclonal |
96T |
EUR 322.37 |
Human Mucin 2(MUC2) ELISA Kit |
|
CSB-E13122h-48T |
Cusabio |
48T |
EUR 385.13 |
|
|
|
Description: Homo sapiens (Human) |
Human Mucin 2(MUC2) ELISA Kit |
|
CSB-E13122h-96T |
Cusabio |
96T |
EUR 592.5 |
|
|
|
Description: Homo sapiens (Human) |
 CLIA Kit) Human Mucin-2 (MUC2) CLIA Kit |
|
20-abx491916 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
|
|
|
Human Mucin-2 (MUC2) CLIA Kit |
|
abx196012-96tests |
Abbexa |
96 tests |
EUR 618.75 |
|
|
 ELISA Kit) Human Mucin-2 (MUC2) ELISA Kit |
|
abx055282-100g |
Abbexa |
100 µg |
Ask for price |
Human Mucin-2 (MUC2) ELISA Kit |
|
abx055282-10g |
Abbexa |
10 µg |
EUR 525 |
Human Mucin-2 (MUC2) ELISA Kit |
|
abx055282-50g |
Abbexa |
50 µg |
Ask for price |
Human Mucin-2 (MUC2) ELISA Kit |
|
abx055282-96tests |
Abbexa |
96 tests |
EUR 848.4 |
|
|
Human Mucin-2 (MUC2) ELISA Kit |
|
20-abx152395 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
|
|
|
Human Mucin-2 (MUC2) ELISA Kit |
|
abx250256-96tests |
Abbexa |
96 tests |
EUR 848.4 |
|
|
Human Mucin-2 (MUC2) ELISA Kit |
|
20-abx572107 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
|
|
|
 Human Mucin-2,MUC2 ELISA KIT |
|
JOT-EK3030Hu |
Jotbody |
96 wells |
Ask for price |
|
Description: Human |
 Human Mucin-2, MUC2 ELISA Kit |
|
MBS166095-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 3460 |
Human Mucin-2, MUC2 ELISA Kit |
|
MBS166095-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 285 |
Human Mucin-2, MUC2 ELISA Kit |
|
MBS166095-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 1750 |
Human Mucin-2, MUC2 ELISA Kit |
|
MBS166095-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 425 |
 Human Mucin-2, MUC2 GENLISA ELISA |
|
KBH3030 |
Krishgen |
1 x 96 wells |
EUR 286 |
 Human MUC2/ Mucin-2 ELISA Kit |
|
E1669Hu |
Sunlong |
1 Kit |
EUR 515 |
|
|
|
Description: SMUC,Intestinal mucin-2 |
 ELISA Kit) Human MUC2(Mucin-2) ELISA Kit |
|
EH1017 |
FN Test |
96T |
EUR 628.92 |
|
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
, partial) Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-EP015222HU |
Cusabio |
1748 mg |
Ask for price |
Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-EP015222HU-20ug |
Cusabio |
20ug |
Ask for price |
|
|
|
Description: 36-240aa |
Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-EP015222HUa0 |
Cusabio |
1749 mg |
Ask for price |
Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-EP015222HUa0-20ug |
Cusabio |
20ug |
Ask for price |
|
|
|
Description: 36-240aa |
,partial) Recombinant Human Mucin-2(MUC2),partial |
|
AP70569 |
SAB |
1mg |
EUR 1978 |
|
|
Recombinant Human Mucin-2(MUC2),partial |
|
AP72691 |
SAB |
each |
Ask for price |
|
|
Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-YP015222HU |
Cusabio |
5856 mg |
Ask for price |
Recombinant Human Mucin-2 (MUC2), partial |
|
CSB-YP015222HU-20ug |
Cusabio |
20ug |
Ask for price |
|
|
|
Description: 36-240aa |
Recombinant Human Mucin-2 (MUC2), partial |
|
MBS958652-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 255 |
Recombinant Human Mucin-2 (MUC2), partial |
|
MBS958652-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 270 |
As the limiting binding accomplice within the heterodimer, the β6 subunit controls αvβ6 expression and availability. Here we describe our understanding of the ITGB6 gene encoding the β6 subunit, together with its construction, transcriptional and post-transcriptional regulation, the organic results noticed in ITGB6 poor mice and medical circumstances of ITGB6 mutations.
